Notch Therapeutics

Role Summary

We are seeking a highly motivated Senior Research Associate in the Protein & Cell Engineering group to play a key role in supporting our cell engineering pipelines. In this position, you will be part of a collaborative team involved in the generation and characterization of genetically modified primary immune cells. You will work with fellow scientists and cross-functional teams to advance Notch’s iPSC-derived T-cell therapy platform.

 

About Notch (www.notchtx.com)

Notch is an early-stage biotech company, working to maximize the benefit of cell therapies through a proprietary T cell-production platform which combines sophisticated product design with commercial-compatible processes to enhance patient outcomes. Notch’s iPSC-based technology platform allows for precision control of notch signaling, which removes several critical limiting factors in the development of cell therapies, delivering the ability to design and manufacture a uniform and unlimited supply of therapeutic T cells.  

 

Key responsibilities:

Support cell engineering pipelines to generate genetically modified cell lines

• Carry out primary human immune cell isolation and mammalian cell culture
• Molecular cloning of DNA vectors for non-viral gene editing and lentiviral vectors
• Lentivirus production and transduction of cells

 

Support the characterization of genetically modified cells

• Carry out molecular biology techniques, including PCR and ddPCR, to evaluate outcomes of gene editing experiments
• Perform immunological assays including multi-color flow cytometry, MSD, and co-culture experiments
• Troubleshoot and optimize protocols for characterization of gene-edited cell lines
• Apply knowledge of T-cell biology and immunology as needed

 

Maintain project timelines and report progress regularly to the project team

• Maintain excellent records of experiments in lab notebooks
• Drive results in multiple projects through effective planning and organization of work

 

Other responsibilities as required

• Present data at group and company meetings and externally as required
• Support lab operations and equipment purchasing/vendor selection

 

Role Requirements and Qualifications

• Bachelor’s degree in molecular biology, bioengineering, cell biology, immunology or a closely related discipline with 6+ years of postgraduate work experience in a biotech or industry position or master’s degree with 3+ years of experience in a biotech/pharma setting
• Demonstrated knowledge and experience working with primary human immune cells, including T-cells
• Experience in mammalian cell culture
• Proficient in running instruments enabling characterization of cell lines such flow cytometers, MSD, Incucyte
• Experience with molecular biology techniques including cloning, PCR, and ddPCR
• Experience with DNA vector design would be a strong advantage
• Experience with CRISPR gene editing systems would be a strong advantage
• Ability to effectively prioritize, plan, and execute next steps based on experimental results in a fast-paced research environment.

 

Desired Characteristics and Demonstrated Skills

• Team-oriented, can-do attitude. Motivated to contribute and to learn new techniques.
• Strong organizational, record-keeping and communication skills, both verbal and written
• Demonstrated ability and keen interest in modelling Notch’s Core Values: Collaborative, Motivated, Ingenious

Salary Range: $87,000 - $112,000

Benefits:

• Paid Vacation and Sick time
  
• Hybrid Work Framework
  
• Healthcare plans
• 401k plan
  
• Commuting Financial Assistance
• Home Office WIFI Support
• Employee assistance programs (EAPs)
• Internal learning & development opportunities 

Notch Therapeutics Inc. is an equal opportunity employer. All qualified applications will receive consideration for employment without regard to race, age, gender identity, sexual orientation, color, religion, sex, marital status, national origin, protected veteran status, disability status, or any other status protected by federal, provincial or local law.   

 

Requests for accommodation can be made at any stage of the recruitment process providing the applicant has met the Bona-fide requirements for the open position. Applicants need to make their requirements known when contacted.